Journal: Advanced Functional Materials
Article Title: Nanoporous, Gas Permeable PEGDA Ink for 3D Printing Organ‐on‐a‐Chip Devices
doi: 10.1002/adfm.202315035
Figure Lengend Snippet: Figure 6. Coculture of cancer and stromal cells in the OoC device. a) 3D confocal microscopy images of tdTomato+ MDA-MB-231 spheroid co-cultured with GFP+ IMR-90 cells at different time points in the OoC platform. b) Depth color map for the height range of 400 μm showing bi-directional migration of cells. c) Confocal microscopy images of GFP+ MDA-MB-231 breast cancer cells spheroid co-cultured with mCherry+ HUVEC cells, demonstrating vessel formation and integration of the cancer spheroid into the vasculature (white and yellow arrows, respectively). d) Schematic of microarray-based proteomic analysis and CXCL12 secretion under different culture conditions, showing increased concentration for OoC co-culture that induces cell migration. Three biological repeats and 20 technical repeats were done for each condition and time course.
Article Snippet: Protein Microarray for CXCL12 Quantification: The CXCL12 antibody microarray was fabricated onto 2D-Aldehyde glass slides (PolyAn, Germany) using a sciFLEXARRAYER SX microarray spotter (Scienion) equipped with a single piezo dispense capillary nozzle with coating 1 (Scienion).
Techniques: Confocal Microscopy, Cell Culture, Migration, Microarray, Concentration Assay, Co-Culture Assay